Overview

  • Product nameAnti-PGC1 alpha antibody
    See all PGC1 alpha primary antibodies
  • Description
    Goat polyclonal to PGC1 alpha
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Sheep, Goat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PGC1 alpha aa 508-520 (internal sequence).
    Sequence:

    C-DGLFDDSEDESDK

  • Positive control
    • Human Skeletal Muscle lysate
  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

Target

  • FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • Tissue specificityHeart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
    Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • L PGC 1alpha antibody
    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1-alpha antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • PRGC1_HUMAN antibody
    see all

Anti-PGC1 alpha antibody images

  • Anti-PGC1 alpha antibody (ab106814) at 0.1 µg/ml + Human Skeletal Muscle Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 91 kDa

    Primary incubation 1 hour

References for Anti-PGC1 alpha antibody (ab106814)

This product has been referenced in:
  • Zou D  et al. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats. PLoS One 10:e0124727 (2015). WB ; Rat . Read more (PubMed: 25919288) »
  • Souza RW  et al. Aerobic exercise training prevents heart failure-induced skeletal muscle atrophy by anti-catabolic, but not anabolic actions. PLoS One 9:e110020 (2014). WB ; Rat . Read more (PubMed: 25330387) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Skeletal muscle
Blocking step Thermo StartingBlock TBS blocking buffer as blocking agent for 30 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2012

Thank you for your enquiry.

By searching for an antibody that is guaranteed to react with rat and to work in WB applications we found the Anti-PGC1 alpha antibody (ab106814). If you click http://www.abcam.com/pgc1-alpha-antibody-ab106814.html ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

ocs detalles.

He revisado consultas anteriores de otros usuarios de este anticuerpo, y no he encontrado ningún caso similar. Si conseguís algún control positivo y lo probáis con las muestras estaría muy bien...

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oc
¿En qué especies estáis usando el anticuerpo? ¿Habéis usado un control positivo? Se recomienda utilizar lisado de estomago de rata. La imagen de la datasheet se realizó con este lisado obtenido con buffe...

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Application Western blot
Sample Rabbit Tissue lysate - whole (aortic tissue)
Loading amount 30 µg
Specification aortic tissue
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: RT°C
Username

Mrs. María Valero

Verified customer

Submitted Feb 16 2012

Lamento la espera.
He recibido una respuesta de mis compañeros del laboratorio respecto al anticuerpo ab105096 (anti-CD36). A pesar de no haberse testado en conejo, es muy posible que reaccione ya que comparten un 85% de homología. S...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"