Overview

  • Product name
  • Description
    Rabbit polyclonal to PGC1 alpha
  • Specificity
    The immunogen sequence for this antibody is 78% homologous with PGC1 beta (Uniprot: Q86YN6).
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Horse, Chicken, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Opossum
  • Immunogen

    Synthetic peptide corresponding to Human PGC1 alpha aa 777-797.
    Sequence:

    C-SKYDSLDFDSLLKEAQRSLRR


    Database link: Q9UBK2

  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

Applications

Our Abpromise guarantee covers the use of ab54481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 92 kDa.

Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low.

ELISA 1/50000.
ICC/IF 1/300. PubMed: 22545021
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • Tissue specificity
    Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
    Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • L PGC 1alpha antibody
    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1-alpha antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • PRGC1_HUMAN antibody
    see all

Anti-PGC1 alpha antibody images

  • All lanes : Anti-PGC1 alpha antibody (ab54481) at 1/2000 dilution

    Lane 1 : Mouse Brown Adipose Tissue at 10 µg
    Lane 2 : Mouse Brown Adipose Tissue at 20 µg


    Predicted band size : 92 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)

    Western blot using ab54481 at 1:2000 to detect PGC1 alpha in Mouse brown adipose tissue whole cell extract (both lanes).

  • Immunofluorescence analysis of Human white adipose tissue progenitor cells, staining PGC1 alpha (green) with ab54481.
    Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 for 5 min. Cells were incubated with primary antibody (1/300) and staining was detected with Alexa Fluor® 546-conjugated anti-rabbit secondary antibody (1/1000).

  • Immunohistochemical analysis of mouse kidney tissue, staining PGC1 alpha with ab54481.

    Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 0.25% protein block for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/250 in 1% BSA + 0.5% skim milk) for 16 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-PGC1 alpha antibody (ab54481)

This product has been referenced in:
  • Le NH  et al. Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle. J Inflamm (Lond) 14:9 (2017). WB ; Mouse . Read more (PubMed: 28503098) »
  • Kodo K  et al. Erythropoietin (EPO) ameliorates obesity and glucose homeostasis by promoting thermogenesis and endocrine function of classical brown adipose tissue (BAT) in diet-induced obese mice. PLoS One 12:e0173661 (2017). WB ; Mouse . Read more (PubMed: 28288167) »

See all 82 Publications for this product

Product Wall

Thank you for your email.

I'm very glad to hear that. Thank you so much for letting me know.

Please do not hesitate to contact meif I can assist you in the future.

Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

Submitted Apr 20 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Wesley Konsavage

Verified customer

Submitted Dec 09 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (anterior tibialis)
Gel Running Conditions
Reduced Denaturing (precast)
Loading amount
50 µg
Specification
anterior tibialis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain section)
Antigen retrieval step
None
Specification
Brain section
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 08 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 16 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Rat Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - nuclear (hepatocytes)
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2014

1-10 of 66 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up