Anti-PGC1 alpha antibody [4A8] (ab77210)
Key features and details
- Mouse monoclonal [4A8] to PGC1 alpha
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-PGC1 alpha antibody [4A8]
See all PGC1 alpha primary antibodies -
Description
Mouse monoclonal [4A8] to PGC1 alpha -
Host species
Mouse -
Specificity
The immunogen sequence for this antibody is 55% homologous with PGC1 beta (Uniprot: Q86YN6). -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human PGC1 alpha aa 689-799.
Database link: Q9UBK2 -
Positive control
- PGC1 alpha transfected U2OS cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
4A8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. -
Tissue specificity
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body. - Information by UniProt
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Database links
- Entrez Gene: 10891 Human
- Omim: 604517 Human
- SwissProt: Q9UBK2 Human
- Unigene: 527078 Human
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Alternative names
- L PGC 1alpha antibody
- LEM6 antibody
- Ligand effect modulator 6 antibody
see all
Images
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Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/ml + Recombinant fragment of human PGC1 alpha at 0.1 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen using ab77210 antibody at 1/500 dilution. Predicted band size of immunogen is 92kDa.
This image was generated using the ascites version of the product.
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Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/ml + PGC1 alpha transfected U2OS cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
Datasheets and documents
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Datasheet download
References (12)
ab77210 has been referenced in 12 publications.
- van Bruggen JAC et al. Depletion of CLL cells by venetoclax treatment reverses oxidative stress and impaired glycolysis in CD4 T cells. Blood Adv 6:4185-4195 (2022). PubMed: 35580333
- Ravera S et al. Identification of Biochemical and Molecular Markers of Early Aging in Childhood Cancer Survivors. Cancers (Basel) 13:N/A (2021). PubMed: 34680366
- Huang WK et al. Heterogeneity of Metabolic Vulnerability in Imatinib -Resistant Gastrointestinal Stromal Tumor. Cells 9:N/A (2020). PubMed: 32466502
- Zhang Z et al. Metabolic reprogramming of normal oral fibroblasts correlated with increased glycolytic metabolism of oral squamous cell carcinoma and precedes their activation into carcinoma associated fibroblasts. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31270582
- Fukushima A et al. Acetylation contributes to hypertrophy-caused maturational delay of cardiac energy metabolism. JCI Insight 3:N/A (2018). PubMed: 29769443