Anti-PGC1 alpha antibody (ab84139)

Overview

  • Product nameAnti-PGC1 alpha antibody
    See all PGC1 alpha primary antibodies
  • Description
    Rabbit polyclonal to PGC1 alpha
  • SpecificityThe immunogen sequence for this antibody is 47% homologous with PGC1 beta (Uniprot: Q86YN6).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within residues 250 and 300 of human PGC1 alpha (SwissProt entry Q9UBK2).

  • Positive control
    • Human stomach adenocarcinoma; human colon adenocarcinoma, ovarian carcinoma
  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

Applications

Our Abpromise guarantee covers the use of ab84139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
IF 1/50 - 1/500.

Target

  • FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • Tissue specificityHeart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
    Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • L PGC 1alpha antibody
    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1-alpha antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • PRGC1_HUMAN antibody
    see all

Anti-PGC1 alpha antibody images

  • ab84139, at a 1/250 dilution, staining PGC1 alpha in formalin fixed, paraffin embedded human stomach adenocarcinoma by Immunohistochemistry.
    Detection: DAB
  • ab84139, at a 1/250 dilution, staining PGC1 alpha in formalin fixed, paraffin embedded human colon adenocarcinoma by Immunohistochemistry.
    Detection: DAB

References for Anti-PGC1 alpha antibody (ab84139)

ab84139 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The immunogen for antibody ab84139 corresponds to a peptide derived from aa 250-300 of human PGC1 and is 98% homologous with the mouse protein.

DISCOUNT CODE: ***
Expiration date: December 2nd, 2012

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Thank you for contacting us.

Please find attached the datasheets in PDF format for the two antibodies that I think may be useful for your study, ab54481 and ab84139. Neither has been tested for IHC on rat tissue, though, and our replacement ...

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This product is prepared to provide specific IHC staining within the recommended range of dilutions.  The concentration of IgG can be assumed to be approximately 1 mg/ml.  I hope this is helpful. Please contact me again if you have any further questions.

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Thank you for contacting us. The alignment sequence between the Zebrafish PGC1alpha (Swiss Prot Q52MY8) and the immunogen of ab54481 shows only 28.6% homology. This antibody is therefore unlikely to recognise the Zebrafish protein, I wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"