Overview

  • Product nameAnti-PGK1 antibody [14]
    See all PGK1 primary antibodies
  • Description
    Mouse monoclonal [14] to PGK1
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein (Human) expressed in E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab67335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA Use at an assay dependent concentration.

Target

  • FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similaritiesBelongs to the phosphoglycerate kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Anti-PGK1 antibody [14] images

  • Anti-PGK1 antibody [14] (ab67335) at 1/1000 dilution + Human recombinant PGK1 protein

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Overlay histogram showing HeLa cells stained with ab67335 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab67335, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-PGK1 antibody [14] (ab67335)

ab67335 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD Perm/Wash Buffer
Sample Human Cell (PBMC)
Specification PBMC
Gating Strategy FSC-A/SSC-A
Preparation Cell harvesting/tissue preparation method: Ficoll isolation of PBMC from whole blood, spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.
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Submitted Jul 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"