The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Use a concentration of 5 - 10 µg/ml.
FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
Sequence similaritiesBelongs to the phosphoglycerate kinase family.
Epididymis secretory sperm binding protein Li 68p antibody
HEL S 68p antibody
Phosphoglycerate kinase 1 antibody
Primer recognition protein 2 antibody
PRP 2 antibody
Anti-PGK1 antibody [22C5D8] images
Western blot - PGK1 antibody [22C5D8] (ab113687)
Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 45 kDa
References for Anti-PGK1 antibody [22C5D8] (ab113687)
This product has been referenced in:
Sen A & Cox RT Clueless is a conserved ribonucleoprotein that binds the ribosome at the mitochondrial outer membrane. Biol Open5:195-203 (2016).
Read more (PubMed: 26834020) »
MacDiarmid CW et al. Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth. J Biol Chem291:18880-96 (2016).
Read more (PubMed: 27432887) »