Overview

  • Product nameAnti-PGK1 antibody [22C5D8]
    See all PGK1 primary antibodies
  • Description
    Mouse monoclonal [22C5D8] to PGK1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified)

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

     

    Alternative versions available:

    Anti-PGK1 antibody (HRP) [22C5D8] (ab197960)

Properties

Applications

Our Abpromise guarantee covers the use of ab113687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similaritiesBelongs to the phosphoglycerate kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • Cell migration-inducing gene 10 protein antibody
      • Epididymis secretory sperm binding protein Li 68p antibody
      • HEL S 68p antibody
      • MGC117307 antibody
      • MGC8947 antibody
      • MIG10 antibody
      • pgk1 antibody
      • PGK1_HUMAN antibody
      • PGKA antibody
      • Phosphoglycerate kinase 1 antibody
      • Primer recognition protein 2 antibody
      • PRP 2 antibody
      see all

    Anti-PGK1 antibody [22C5D8] images

    • Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 45 kDa

    References for Anti-PGK1 antibody [22C5D8] (ab113687)

    This product has been referenced in:
    • Sen A & Cox RT Clueless is a conserved ribonucleoprotein that binds the ribosome at the mitochondrial outer membrane. Biol Open 5:195-203 (2016). Read more (PubMed: 26834020) »
    • MacDiarmid CW  et al. Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth. J Biol Chem 291:18880-96 (2016). Read more (PubMed: 27432887) »

    See all 13 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"