Overview

  • Product nameAnti-PGK1 antibody
    See all PGK1 primary antibodies
  • Description
    Rabbit polyclonal to PGK1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH selected within aa 100~200 of human PGK1.

  • Positive control
    • HepG2 cell lysate. Mouse stomach tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab38007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.

Target

  • FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similaritiesBelongs to the phosphoglycerate kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Anti-PGK1 antibody images

  • All lanes : Anti-PGK1 antibody (ab38007) at 1/100 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : HepG2 cell lysate


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab38007 at 1/100 dilution staining PGK1 in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ICC/IF image of ab38007 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PGK1 antibody (ab38007)

This product has been referenced in:
  • Fossat N  et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep N/A:N/A (2014). Read more (PubMed: 24916387) »
  • Chaput CD  et al. A proteomic study of protein variation between osteopenic and age-matched control bone tissue. Exp Biol Med (Maywood) 237:491-8 (2012). WB ; Human . Read more (PubMed: 22619369) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted May 26 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - whole cell (Whole cell lysate)
Loading amount 100 µg
Specification Whole cell lysate
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"