• Product nameAnti-PGK1 antibody
    See all PGK1 primary antibodies
  • Description
    Rabbit polyclonal to PGK1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH selected within aa 100~200 of human PGK1.

  • Positive control
    • HepG2 cell lysate. Mouse stomach tissue lysate.


Associated products


Our Abpromise guarantee covers the use of ab38007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.


  • FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similaritiesBelongs to the phosphoglycerate kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Anti-PGK1 antibody images

  • All lanes : Anti-PGK1 antibody (ab38007) at 1/100 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : HepG2 cell lysate

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab38007 at 1/100 dilution staining PGK1 in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ICC/IF image of ab38007 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PGK1 antibody (ab38007)

This product has been referenced in:
  • Fossat N  et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep N/A:N/A (2014). Read more (PubMed: 24916387) »
  • Chaput CD  et al. A proteomic study of protein variation between osteopenic and age-matched control bone tissue. Exp Biol Med (Maywood) 237:491-8 (2012). WB ; Human . Read more (PubMed: 22619369) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 26 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - whole cell (Whole cell lysate)
Loading amount 100 µg
Specification Whole cell lysate
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 02 2010