Overview

  • Product name
  • Description
    Rabbit polyclonal to PGK1
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the center region of human PGK1 conjugated to KLH

  • Positive control
    • Hela cell line lysate. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

Target

  • Function
    In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in disease
    Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similarities
    Belongs to the phosphoglycerate kinase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Images

  • Anti-PGK1 antibody (ab71257) at 1/60 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab71257 at 1/10 dilution, staining PGK1 in human hepatocarcinoma by Immunohistochemistry using Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab71257 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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