Recombinant
RabMAb

Anti-PGM1 antibody [EPR15240] (ab188869)

Overview

  • Product name
    Anti-PGM1 antibody [EPR15240]
    See all PGM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15240] to PGM1
  • Tested applications
    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGM1 aa 1-100. The exact sequence is proprietary.
    Database link: P36871

  • Positive control
    • Human fetal heart, fetal liver, HepG2, 293T and Jurkat lysates; 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab188869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Flow Cyt 1/360. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/30 - 1/50.
ICC/IF 1/250.

Target

  • Function
    This enzyme participates in both the breakdown and synthesis of glucose.
  • Involvement in disease
    Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14) [MIM:612934]. A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test.
  • Sequence similarities
    Belongs to the phosphohexose mutase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDG1T antibody
    • Glucose phosphomutase 1 antibody
    • GSD14 antibody
    • OTTHUMP00000010519 antibody
    • OTTHUMP00000010520 antibody
    • PGM 1 antibody
    • PGM1 antibody
    • PGM1_HUMAN antibody
    • Phosphoglucomutase 1 antibody
    • Phosphoglucomutase-1 antibody
    • Phosphoglucomutase1 antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 61 kDa
  • All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : 293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size : 61 kDa

References

ab188869 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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