Overview

  • Product nameAnti-PGP antibody
  • Description
    Rabbit polyclonal to PGP
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 40-70 of Human PGP

  • Positive control
    • K562 lysate, Human skeletal muscle This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab103477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Tissue specificityDetected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • PGP antibody
    • PGP_HUMAN antibody
    • PGPase antibody
    • Phosphoglycolate phosphatase antibody

Anti-PGP antibody images

  • Anti-PGP antibody (ab103477) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 34 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab103477 at a dilution of 1/50.
  • ICC/IF image of ab103477 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103477 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PGP antibody (ab103477)

This product has been referenced in:
  • Zhao H  et al. HDAC2 overexpression is a poor prognostic factor of breast cancer patients with increased multidrug resistance-associated protein expression who received anthracyclines therapy. Jpn J Clin Oncol 46:893-902 (2016). Read more (PubMed: 27432453) »

See 1 Publication for this product

Product Wall

Malheureusement, comme le montre l'alignement de séquence (voir fichier jpg ci-joint) l'anticorps anti-PGP référence ab103477 n'est pas adapté à la détection de la PGP issue de Dreissena polymorpha. Peut-être aurez-vous de meilleures chances de trouv...

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Comme expliqué par téléphone, pouvez-vous, s'il vous plait, m'envoyer la séquence de la PGP d'invertébré aquatique afin de déterminer si l'anticorps pyclonal de lapin anti-PGP ab103477 a des chances de fonctionner dans cette espèce non-testée. Un ex...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"