Overview

  • Product nameAnti-PGP9.5 antibody [13C4 / I3C4]
    See all PGP9.5 primary antibodies
  • Description
    Mouse monoclonal [13C4 / I3C4] to PGP9.5
  • SpecificityThis antibody reacts with human neuroendocrine marker PGP 9.5.
  • Tested applicationsICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Dog, Human, Pig, Zebrafish
  • Immunogen

    PGP 9.5 from human brain.

  • Positive control
    • In Western Blot, ab8189 gave a positive signal in the following lysates: human, mouse and rat brain tissue; rat cortex tissue; human spinal cord tissue; SHSY5Y whole cell. This antibody also gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.
  • General notes

    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.

     

    Alternative versions available:

    Anti-PGP9.5 antibody (Alexa Fluor® 488) [13C4 / I3C4] (ab197733)

    Anti-PGP9.5 antibody (HRP) [13C4 / I3C4] (ab197735)

Properties

Applications

Our Abpromise guarantee covers the use of ab8189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50.
ICC/IF 1/20 - 1/50.
IHC-FoFr 1/10 - 1/20.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseParkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gracile axonal dystrophy antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • Parkinson Disease 5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase (neuron specific) antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody [13C4 / I3C4] images

  • ab8189 (1/20) immunostaining neurons in mouse cortical primary cell culture.
  • IHC-P image of PGP9.5 staining on zebrafish brain using ab8189 (1:1000). The sections were subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins for 21°C. The primary antibody (ab8189) was incubated at a dilution of 1:1000 at 21°C for 16 hours. The secondary antibody used was undiluted goat polyclonal to Mouse IgG conjugated to biotin.

    See Abreview

  • IHC-P image of PGP9.5 staining on P5 mouse skin sections using ab8189 (1:1000). The sections were de paraffinized and subjected to heat meadiated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab8189 was then incubated for 16 hours at 21°C. the secondary antibidy used was Got polyclonal to anti mouse IgG conjugated to biotin (1:200).

    See Abreview

  • IHC image of PGP9.5 staining in Rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) at 5 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa


    Exposure time : 1 minute

References for Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

This product has been referenced in:
  • Beeken M  et al. Alterations in the Ubiquitin Proteasome System in Persistent but Not Reversible Proteinuric Diseases. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24722446) »
  • Fernandes VS  et al. Hydrogen sulfide plays a key role in the inhibitory neurotransmission to the pig intravesical ureter. PLoS One 9:e113580 (2014). IHC-Fr ; Pig . Read more (PubMed: 25415381) »

See all 23 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 23 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (SHSY-5Y)
Specification SHSY-5Y
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (brain, spinal cord)
Specification brain, spinal cord
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample Human Tissue sections (Nerve)
Specification Nerve
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount 30 µg
Specification Sciatic nerve
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted May 14 2013

Thank you for contacting us.


Both of these targets should be general neuronal markers according to the literature. We have not done any testing in mouse gut.


I hope this information is helpful to you. Please do not hesitate ...

Read More

Thank you for contacting us.

According to UniGene, the RNA for this protein can be found quite abundantly in human pancreas. Therefore, it shouldn't be a problem to find a suitable antibody which has been tested in human and IHC-P.

Read More

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (Bladder)
Specification Bladder
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (P5 Skin)
Specification P5 Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Zebrafish Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"