Overview

  • Product nameAnti-PGP9.5 antibody [13C4 / I3C4]See all PGP9.5 primary antibodies ...
  • Description
    Mouse monoclonal [13C4 / I3C4] to PGP9.5
  • SpecificityThis antibody reacts with human neuroendocrine marker PGP 9.5.
  • Tested applicationsICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Dog, Human, Pig, Zebrafish
  • Immunogen

    PGP 9.5 from human brain.

  • Positive control
    • In Western Blot, ab8189 gave a positive signal in the following lysates: human, mouse and rat brain tissue; rat cortex tissue; human spinal cord tissue; SHSY5Y whole cell. This antibody also gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.
  • General notes


    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab8189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ICC/IF
IHC-FoFr
IHC-Fr
IHC-P
WB
  • Application notesICC: 1/50.
    ICC/IF: 1/20 - 1/50.
    IHC-FoFr: 1/10 - 1/20.
    IHC-P: 1/10 - 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
    • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
    • Sequence similaritiesBelongs to the peptidase C12 family.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gracile axonal dystrophy antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • Parkinson Disease 5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase (neuron specific) antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Anti-PGP9.5 antibody [13C4 / I3C4] images

    • ab8189 (1/20) immunostaining neurons in mouse cortical primary cell culture.
    • IHC-P image of PGP9.5 staining on zebrafish brain using ab8189 (1:1000). The sections were subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins for 21°C. The primary antibody (ab8189) was incubated at a dilution of 1:1000 at 21°C for 16 hours. The secondary antibody used was undiluted goat polyclonal to Mouse IgG conjugated to biotin.

      See Abreview

    • IHC-P image of PGP9.5 staining on P5 mouse skin sections using ab8189 (1:1000). The sections were de paraffinized and subjected to heat meadiated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab8189 was then incubated for 16 hours at 21°C. the secondary antibidy used was Got polyclonal to anti mouse IgG conjugated to biotin (1:200).

      See Abreview

    • IHC image of PGP9.5 staining in Rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) at 5 µg/ml

      Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : Rat Cortex Tissue Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 6 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 25 kDa
      Observed band size : 25 kDa


      Exposure time : 1 minute

    References for Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

    This product has been referenced in:
    • Beeken M  et al. Alterations in the Ubiquitin Proteasome System in Persistent but Not Reversible Proteinuric Diseases. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24722446) »
    • Bakovic M  et al. Changes in cardiac innervation during maturation in long-term diabetes. Exp Gerontol 48:1473-8 (2013). Rat . Read more (PubMed: 24140619) »

    See all 22 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
    Sample Human Tissue sections (Nerve)
    Specification Nerve
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted May 28 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Sciatic nerve)
    Loading amount 30 µg
    Specification Sciatic nerve
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. G.K Sakellariou

    Verified customer

    Submitted May 14 2013

    Thank you for contacting us.


    Both of these targets should be general neuronal markers according to the literature. We have not done any testing in mouse gut.


    I hope this information is helpful to you. Please do not hesitate ...

    Read More

    Thank you for contacting us.

    According to UniGene, the RNA for this protein can be found quite abundantly in human pancreas. Therefore, it shouldn't be a problem to find a suitable antibody which has been tested in human and IHC-P.

    Read More

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cat Tissue sections (Bladder)
    Specification Bladder
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (P5 Skin)
    Specification P5 Skin
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 16 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Zebrafish Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 16 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibroblasts)
    Specification fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,1%Saponin/PBS
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 11 2012

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 14 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (skin)
    Loading amount 30 µg
    Specification skin
    Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"