Overview

  • Product name
    Anti-PGP9.5 antibody [13C4 / I3C4]
    See all PGP9.5 primary antibodies
  • Description
    Mouse monoclonal [13C4 / I3C4] to PGP9.5
  • Specificity
    This antibody reacts with human neuroendocrine marker PGP 9.5.
  • Tested applications
    Suitable for: ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Dog, Human, Pig, Zebrafish
  • Immunogen

    PGP 9.5 from human brain.

  • Positive control
    • In Western Blot, ab8189 gave a positive signal in the following lysates: human, mouse and rat brain tissue; rat cortex tissue; human spinal cord tissue; SHSY5Y whole cell. This antibody also gave a positive result in IHC in the following FFPE tissue: Rat normal pancreas.
  • General notes

    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.

     

    Alternative versions available:

    Anti-PGP9.5 antibody (Alexa Fluor® 488) [13C4 / I3C4] (ab197733)

    Anti-PGP9.5 antibody (HRP) [13C4 / I3C4] (ab197735)

Properties

Applications

Our Abpromise guarantee covers the use of ab8189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50.
ICC/IF 1/20 - 1/50.
IHC-FoFr 1/10 - 1/20.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity
    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease
    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities
    Belongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody [13C4 / I3C4] images

  • IHC-P image of PGP9.5 staining on P5 mouse skin sections using ab8189 (1:1000). The sections were de paraffinized and subjected to heat meadiated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab8189 was then incubated for 16 hours at 21°C. the secondary antibidy used was Got polyclonal to anti mouse IgG conjugated to biotin (1:200).

    See Abreview

  • All lanes : Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) at 5 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : Human spinal cord tissue lysate - total protein (ab29188)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa


    Exposure time : 1 minuteThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab8189 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • ab8189 (1/20) immunostaining neurons in mouse cortical primary cell culture.
  • IHC-P image of PGP9.5 staining on zebrafish brain using ab8189 (1:1000). The sections were subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins for 21°C. The primary antibody (ab8189) was incubated at a dilution of 1:1000 at 21°C for 16 hours. The secondary antibody used was undiluted goat polyclonal to Mouse IgG conjugated to biotin.

    See Abreview

  • IHC image of PGP9.5 staining in Rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

This product has been referenced in:
  • Takahashi H  et al. A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors. J Neurosci 36:8210-27 (2016). Read more (PubMed: 27488640) »
  • Arosh JA  et al. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci U S A 112:9716-21 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26199416) »

See all 26 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 24 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Small intestine
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 16 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Sciatic nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 23 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (SHSY-5Y)
Specification
SHSY-5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (brain, spinal cord)
Specification
brain, spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Nerve)
Specification
Nerve
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount
30 µg
Specification
Sciatic nerve
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted May 14 2013

Thank you for contacting us.


Both of these targets should be general neuronal markers according to the literature. We have not done any testing in mouse gut.


I hope this information is helpful to you. Please do not hesitate ...

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