Overview

  • Product nameAnti-PGP9.5 antibody
    See all PGP9.5 primary antibodies
  • Description
    Rabbit polyclonal to PGP9.5
  • Tested applicationsIHC-Fr, WB, IHC-P, IHC (PFA fixed), ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide:

    ASSEDTLLKDAAKVCR

    , corresponding to amino acids 176 - 191 of Human PGP9.5.

  • Positive control
    • Human brain lysate.
  • General notesSerum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab10404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000.
WB 1/1000. Predicted molecular weight: 26.8 kDa.Can be blocked with Human PGP9.5 peptide (ab38203).
IHC-P 1/100 - 1/300.
IHC (PFA fixed) 1/200. PubMed: 17508253
ICC Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

Anti-PGP9.5 antibody images

  • Immunofluorescence analysis of mouse gonocytes, staining PGP9.5 with ab10404.

    Cells were permabilized with 0.025% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Cells were incubated with primary antibody (1/20) overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.
  • ab10404 at a 1/500 dilution staining human cancer prostate tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Following heat mediated antigen retrieval the tissue was fixed with formaldehyde and blocked with 20% serum. The tissue was incubated with the primary antibody for 1 hour and then with an HRP conjugated rabbit polyclonal antibody.

    See Abreview

  • Anti-PGP9.5 antibody (ab10404) at 1/1000 dilution + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 26.8 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,70 kDa,73 kDa. We are unsure as to the identity of these extra bands.
  • ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30µm thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • ab10404 staining PGP9.5 in Mouse Endometriotic tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 3% BSA) for 20 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).

References for Anti-PGP9.5 antibody (ab10404)

This product has been referenced in:
  • Zhu X  et al. Gpr177-mediated Wnt Signaling is Required for Fungiform Placode Initiation. J Dent Res 93:582-588 (2014). IHC-Fr . Read more (PubMed: 24736288) »
  • Kosacka J  et al. Increased autophagy in peripheral nerves may protect Wistar Ottawa Karlsburg W rats against neuropathy. Exp Neurol 250:125-35 (2013). IHC-FoFr ; Rat . Read more (PubMed: 24095727) »

See all 11 Publications for this product

Product Wall

Thank you for your question. The protein PGP9.5 is expressed in neurons and most brain tissue, especially from the cerebral cortex, will be a good positive tissue control. A negative tissue control is more difficult to recommend but the  Human Protein ...

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Thank you for contacting us.

According to UniGene, the RNA for this protein can be found quite abundantly in human pancreas. Therefore, it shouldn't be a problem to find a suitable antibody which has been tested in human and IHC-P.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Endometriotic tissue)
Specification Endometriotic tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Brett McKinnon

Verified customer

Submitted Jan 15 2013

Thank you for contacting us.

ab10404 is a whole serum product and the specific antibody concentration cannot be determined.

I am sorry for not being able to provide useful information this time. Please do not hesitate to contact u...

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Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this ca...

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TThank you for calling Abcam regarding positive controls.

AB10404 has been used in IHC-P on lung sections in the following publication:Liu Yet al.Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (WERI-RB27 Human Retinoblastoma Cells)
Specification WERI-RB27 Human Retinoblastoma Cells
Fixative Cytospin solution
Permeabilization Yes - Tween-PBS
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human cancer tissue, prostate)
Specification human cancer tissue, prostate
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20%
Username

Abcam user community

Verified customer

Submitted Jul 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"