Overview

  • Product nameAnti-PGP9.5 antibody
    See all PGP9.5 primary antibodies
  • Description
    Goat polyclonal to PGP9.5
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide (

    C-QHENFRKKQIE

    ) corresponding to internal sequence amino acids 58-68 of Human PGP9.5 (NP_004172.2).

  • Positive control
    • Human Brain (Cerebellum and Hippocampus) and Rat Brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab104938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody images

  • All lanes : Anti-PGP9.5 antibody (ab104938) at 0.00999999977648 µg/ml

    Lane 1 : Mouse Brain Lysates in RIPA buffer
    Lane 2 : Rat Brain Lysates in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    developed using the ECL technique

    Predicted band size : 25 kDa
  • Anti-PGP9.5 antibody (ab104938) at 0.01 µg/ml (for 1 hour) + Human Hippocampus lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : ~26 kDa (why is the actual band size different from the predicted?)

References for Anti-PGP9.5 antibody (ab104938)

ab104938 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"