The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/500.
WB: 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). The UCHL1 protein runs at about 24kDa on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Immunofluorescent staining of rat mixed neuron/glial cultures stained with ab64720 (1:500, green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.
References for Anti-PGP9.5 antibody (ab64720)
has not yet been referenced specifically in any publications.
Publishing research using ab64720? Please let us know so that we can cite the reference in this datasheet.
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