Overview

  • Product nameAnti-PGP9.5 antibodySee all PGP9.5 primary antibodies ...
  • Description
    Chicken polyclonal to PGP9.5
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length human PGP9.5 purified from E. coli.

  • Positive control
    • Rat hippocampal homogenate and Rat mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab72910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/500.

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gracile axonal dystrophy antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • Parkinson Disease 5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase (neuron specific) antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH L1 antibody
    • UCH-L1 antibody
    • UCHL 1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody images

  • Anti-PGP9.5 antibody (ab72910) at 1/2500 dilution + rat hippocampal homogenate

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.

References for Anti-PGP9.5 antibody (ab72910)

This product has been referenced in:
  • Fu YY  et al. 3-D imaging and illustration of mouse intestinal neurovascular complex. Am J Physiol Gastrointest Liver Physiol 304:G1-11 (2013). ICC/IF ; Mouse . Read more (PubMed: 23086917) »
  • Allen C  et al. Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA. J Immunol 189:381-92 (2012). Read more (PubMed: 22661091) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call. For the neuronal cell body marker, I would recommend one of our chicken polyclonal to PGP9.5 antibodies: ab63497 or ab72910.

DISCOUNT CODE: ***
Expiration date: October 8th, 2012

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human skin)
Specification human skin
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.3 %
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"