Overview

  • Product nameAnti-PGP9.5 antibody [BH7]See all PGP9.5 primary antibodies ...
  • Description
    Mouse monoclonal [BH7] to PGP9.5
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Recombinant full length human UCHL1 purified from E. coli.

  • Positive control
    • Rat spinal cord, brain, SHSY-5Y, HEK293 cell extract or whole cell homogenate of bovine brain.
  • General notesThis antibody is made in an Integra CL350 flask, with total protein content about 10mg/ml, of which about 1mg/ml is antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab64722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/2000.

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Gracile axonal dystrophy antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • Parkinson Disease 5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase (neuron specific) antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody [BH7] images

  • Lanes 1 - 2 : A monoclonal antibody reactive with PGP9.5
    Lane 3 : Anti-PGP9.5 antibody [BH7] (ab64722) at 1/10000 dilution

    Lane 1 : Whole cell homogenate of bovine brain
    Lane 2 : Whole cell homogenate of bovine brain
    Lane 3 : Whole cell homogenate of bovine brain


    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of rat spinal cord with ab64722 (1:1000, red). These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.

References for Anti-PGP9.5 antibody [BH7] (ab64722)

ab64722 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab64722.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"