Anti-PGP9.5 antibody [BH7] (ab64722)
- Product nameAnti-PGP9.5 antibody [BH7]See all PGP9.5 primary antibodies ...
- DescriptionMouse monoclonal [BH7] to PGP9.5
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Rat, Cow, Human
Recombinant full length human UCHL1 purified from E. coli.
- Positive control
- Rat spinal cord, brain, SHSY-5Y, HEK293 cell extract or whole cell homogenate of bovine brain.
- General notesThis antibody is made in an Integra CL350 flask, with total protein content about 10mg/ml, of which about 1mg/ml is antibody.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 10mM Sodium Azide
Constituents: Tissue culture supernatant
- Concentration information loading...
- Purification notesAscites fluid
- Clonality Monoclonal
- Clone numberBH7
- Research Areas
Our Abpromise guarantee covers the use of ab64722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).|
- FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
- Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
- Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
- Sequence similaritiesBelongs to the peptidase C12 family.
- Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
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Anti-PGP9.5 antibody [BH7] images
Lanes 1 - 2 : A monoclonal antibody reactive with PGP9.5
Lane 3 : Anti-PGP9.5 antibody [BH7] (ab64722) at 1/10000 dilution
Lane 1 : Whole cell homogenate of bovine brain
Lane 2 : Whole cell homogenate of bovine brain
Lane 3 : Whole cell homogenate of bovine brain
Predicted band size : 25 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Immunofluorescent staining of rat spinal cord with ab64722 (1:1000, red). These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
References for Anti-PGP9.5 antibody [BH7] (ab64722)
ab64722 has not yet been referenced specifically in any publications.