RabMAb

Anti-PGP9.5 antibody [EPR4117] (ab109261)

Overview

  • Product nameAnti-PGP9.5 antibody [EPR4117]
    See all PGP9.5 primary antibodies
  • Description
    Rabbit monoclonal [EPR4117] to PGP9.5
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human PGP9.5.

  • Positive control
    • Fetal brain, Y79, U-87 MG, and SH SY5Y lysates; Human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
    • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
    • Sequence similaritiesBelongs to the peptidase C12 family.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Anti-PGP9.5 antibody [EPR4117] images

    • All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Y79 lysate
      Lane 3 : U-87 MG lysate
      Lane 4 : SH SY5Y lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 24 kDa
    • ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

      Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
      Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
      Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)

      Lysates/proteins at 100 µg per lane.

      Secondary
      HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution
      developed using the ECL technique

      Predicted band size : 24 kDa


      Exposure time : 2 minutes

      Image courtesy of Dr Simone Diestel by Abreview.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-PGP9.5 antibody [EPR4117] (ab109261)

    ab109261 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Purified protein (Lung)
    Loading amount 50 µg
    Specification Lung
    Gel Running Conditions Reduced Denaturing (10 % gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Mr. Bon-Kwan Goo

    Verified customer

    Submitted Feb 26 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (adult brain)
    Loading amount 100 µg
    Specification adult brain
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Simone Diestel

    Verified customer

    Submitted Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"