Anti-PGP9.5 antibody [EPR4118] (ab108986)
- Product nameAnti-PGP9.5 antibody [EPR4118]See all PGP9.5 primary antibodies ...
- DescriptionRabbit monoclonal [EPR4118] to PGP9.5
- Tested applicationsICC/IF, WB, IP, IHC-P, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human PGP9.5 (UniProt P09936).
- Positive control
- WB: Fetal brain, Y79, U87-MG, SH-SY5Y and 293T cell lysates IHC-P: Human glioma tissue
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4118
- Research Areas
Our Abpromise guarantee covers the use of ab108986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration. PubMed: 23086917|
|WB||WB: 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).|
|IP||IP: 1/10 - 1/100.|
|IHC-P||IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.|
|Flow Cyt||Flow Cyt: 1/10 - 1/100.|
- FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
- Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
- Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
- Sequence similaritiesBelongs to the peptidase C12 family.
- Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
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Anti-PGP9.5 antibody [EPR4118] images
All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/1000 dilution
Lane 1 : Fetal brain cell lysate
Lane 2 : Y79 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size : 24 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Immunohistochemical staining of PGP9.5 in paraffin embedded Human glioma tissue, using ab108986 at a 1/250 dilution.
References for Anti-PGP9.5 antibody [EPR4118] (ab108986)
This product has been referenced in:
- Fu YY et al. 3-D imaging and illustration of mouse intestinal neurovascular complex. Am J Physiol Gastrointest Liver Physiol 304:G1-11 (2013). ICC/IF ; Mouse . Read more (PubMed: 23086917) »
- Liu YA et al. Optical clearing improves the imaging depth and signal-to-noise ratio for digital analysis and three-dimensional projection of the human enteric nervous system. Neurogastroenterol Motil 23:e446-57 (2011). Read more (PubMed: 21895876) »