Anti-PGP9.5 antibody - Neuronal Marker (ab10404)

Overview

  • Product nameAnti-PGP9.5 antibody - Neuronal MarkerSee all PGP9.5 primary antibodies ...
  • Description
    Rabbit polyclonal to PGP9.5 - Neuronal Marker
  • Tested applicationsIHC-Fr, WB, IHC-P, IHC (PFA fixed), ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide:

    ASSEDTLLKDAAKVCR

    , corresponding to amino acids 176 - 191 of Human PGP9.5.

  • Positive control
    • Human brain lysate.
  • General notesSerum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.

Properties

Applications

Our Abpromise guarantee covers the use of ab10404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000.
WB 1/1000. Predicted molecular weight: 26.8 kDa.Can be blocked with Human PGP9.5 peptide (ab38203).
IHC-P 1/100 - 1/300.
IHC (PFA fixed) 1/200. PubMed: 17508253
ICC Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gracile axonal dystrophy antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • Park5 antibody
    • Parkinson Disease 5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase (neuron specific) antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody - Neuronal Marker images

  • ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30µm thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • ab10404 at a 1/500 dilution staining human cancer prostate tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Following heat mediated antigen retrieval the tissue was fixed with formaldehyde and blocked with 20% serum. The tissue was incubated with the primary antibody for 1 hour and then with an HRP conjugated rabbit polyclonal antibody.

    See Abreview

  • ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).
  • Anti-PGP9.5 antibody - Neuronal Marker (ab10404) at 1/1000 dilution + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 26.8 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,70 kDa,73 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of mouse gonocytes, staining PGP9.5 with ab10404.

    Cells were permabilized with 0.025% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Cells were incubated with primary antibody (1/20) overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.

References for Anti-PGP9.5 antibody - Neuronal Marker (ab10404)

This product has been referenced in:
  • Zhu X  et al. Gpr177-mediated Wnt Signaling is Required for Fungiform Placode Initiation. J Dent Res 93:582-588 (2014). IHC-Fr . Read more (PubMed: 24736288) »
  • Kosacka J  et al. Increased autophagy in peripheral nerves may protect Wistar Ottawa Karlsburg W rats against neuropathy. Exp Neurol 250:125-35 (2013). IHC-FoFr ; Rat . Read more (PubMed: 24095727) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.

According to UniGene, the RNA for this protein can be found quite abundantly in human pancreas. Therefore, it shouldn't be a problem to find a suitable antibody which has been tested in human and IHC-P.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Endometriotic tissue)
Specification Endometriotic tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Brett McKinnon

Verified customer

Submitted Jan 15 2013

Thank you for contacting us.

ab10404 is a whole serum product and the specific antibody concentration cannot be determined.

I am sorry for not being able to provide useful information this time. Please do not hesitate to contact u...

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Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

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TThank you for calling Abcam regarding positive controls.

AB10404 has been used in IHC-P on lung sections in the following publication:Liu Yet al.Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (WERI-RB27 Human Retinoblastoma Cells)
Specification WERI-RB27 Human Retinoblastoma Cells
Fixative Cytospin solution
Permeabilization Yes - Tween-PBS
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human cancer tissue, prostate)
Specification human cancer tissue, prostate
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20%
Username

Abcam user community

Verified customer

Submitted Jul 28 2006

Thank you for your phone call and I apologize for the shortage that you received in your vial of ab10404. I have arranged for a free of charge replacement vial to be sent to you. It is on order# 95966 and you should receive this early next week. Yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"