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Synthetic peptide: ASSEDTLLKDAAKVCR, corresponding to amino acids 176 - 191 of Human PGP9.5.
Our Abpromise guarantee covers the use of ab10404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)|
ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"