Anti-PGP9.5 antibody - Neuronal Marker (ab10404)
- Product nameAnti-PGP9.5 antibody - Neuronal MarkerSee all PGP9.5 primary antibodies ...
- DescriptionRabbit polyclonal to PGP9.5 - Neuronal Marker
- Tested applicationsIHC-Fr, WB, IHC-P, IHC (PFA fixed), ICC, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Horse
Synthetic peptide: ASSEDTLLKDAAKVCR, corresponding to amino acids 176 - 191 of Human PGP9.5.
- Positive control
- Human brain lysate.
- General notesSerum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: Whole Serum
- PurityWhole antiserum
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab10404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)|
IHC-P: 1/100 - 1/300.
Immunohistochemistry (PFA fixed): 1/200 (from PMID 17508253)
WB: 1/1000. Predicted molecular weight: 26.8 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
- Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
- Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
- Sequence similaritiesBelongs to the peptidase C12 family.
- Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Entrez Gene: 100033838 Horse
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 396637 Pig
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: Q9GM50 Horse
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- SwissProt: Q6SEG5 Pig
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
- Unigene: 29807 Mouse
- Unigene: 107213 Rat
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Anti-PGP9.5 antibody - Neuronal Marker images
ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
µm thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab10404 at a 1/500 dilution staining human cancer prostate tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Following heat mediated antigen retrieval the tissue was fixed with formaldehyde and blocked with 20% serum. The tissue was incubated with the primary antibody for 1 hour and then with an HRP conjugated rabbit polyclonal antibody.
ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).
Anti-PGP9.5 antibody - Neuronal Marker (ab10404) at 1/1000 dilution +
Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 26.8 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa,70 kDa,73 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of mouse gonocytes, staining PGP9.5 with ab10404.
Cells were permabilized with 0.025% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Cells were incubated with primary antibody (1/20) overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.
References for Anti-PGP9.5 antibody - Neuronal Marker (ab10404)
This product has been referenced in:
- McIver SC et al. A unique combination of male germ cell miRNAs coordinates gonocyte differentiation. PLoS One 7:e35553 (2012). ICC/IF ; Mouse . Read more (PubMed: 22536405) »
- Gazerani P et al. Sensitization of rat facial cutaneous mechanoreceptors by activation of peripheral N-methyl-d-aspartate receptors. Brain Res 1319:70-82 (2010). IHC-Fr ; Rat . Read more (PubMed: 20080077) »