Anti-PGP9.5 antibody - Neuronal Marker (ab10410)
- Product nameAnti-PGP9.5 antibody - Neuronal MarkerSee all PGP9.5 primary antibodies ...
- DescriptionGuinea pig polyclonal to PGP9.5 - Neuronal Marker
- Tested applicationsIHC-Fr, IHC-FoFr, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Macaque Monkey
Synthetic peptide: GASSEDTLLKDAAKVCL, corresponding to amino acids 175-191 of soluble cytoplasmic protein PGP9.5, human.
- Positive control
- IF: DU145 cell line
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: Whole Serum
- PurityWhole antiserum
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab10410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||IHC-FoFr: Use at an assay dependent dilution.|
- FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
- Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
- Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
- Sequence similaritiesBelongs to the peptidase C12 family.
- Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
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Anti-PGP9.5 antibody - Neuronal Marker images
ab10410, at a dilution of 1/500, staining PGP9.5 in rat submucous plexus by Immunohistochemistry (PFA perfusion fixed frozen sections).
ICC/IF image of ab10410 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10410, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96959, DyLight® 488 goat anti-guinea pig IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PGP9.5 antibody - Neuronal Marker (ab10410)
This product has been referenced in:
- Buckinx R et al. Transient receptor potential vanilloid type 1 channel (TRPV1) immunolocalization in the murine enteric nervous system is affected by the targeted C-terminal epitope of the applied antibody. J Histochem Cytochem 61:421-32 (2013). Read more (PubMed: 23482327) »