Overview

  • Product nameAnti-PGRMC1 antibody
    See all PGRMC1 primary antibodies
  • Description
    Goat polyclonal to PGRMC1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Macaque Monkey
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    C-EPKDESARKND

    , corresponding to C terminal amino acids 185-195 of Human PGRMC1.

  • Positive control
    • Human kidney, liver and testis lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab48012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.33 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 22307145

Target

Anti-PGRMC1 antibody images

  • Anti-PGRMC1 antibody (ab48012) at 0.1 µg/ml + Human Kidney lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab48012 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48012, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PGRMC1 antibody (ab48012)

This product has been referenced in:
  • Peluso JJ  et al. Evidence for a genomic mechanism of action for progesterone receptor membrane component-1. Steroids 77:1007-12 (2012). WB ; Human . Read more (PubMed: 22326699) »
  • Keator CS  et al. Alterations in progesterone receptor membrane component 2 (PGRMC2) in the endometrium of macaques afflicted with advanced endometriosis. Mol Hum Reprod 18:308-19 (2012). IHC-P ; Macaque Monkey . Read more (PubMed: 22307145) »

See all 5 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"