• Product nameAnti-PGRMC1 antibody
    See all PGRMC1 primary antibodies
  • Description
    Rabbit polyclonal to PGRMC1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PGRMC1.

    (Peptide available as ab88948.)

  • Positive control
    • This antibody gave a positive signal in Human Ovary tissue lysate and the following whole cell lysates: T47D; MCF7; HEK293 and HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.


Associated products


Our Abpromise guarantee covers the use of ab88381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.


Anti-PGRMC1 antibody images

  • IHC image of PGRMC1 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88381, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-PGRMC1 antibody (ab88381) at 1 µg/ml

    Lane 1 : T47D whole cell lysate (ab14899)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,250 kDa,300 kDa,58 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    Membrane-associated progesterone receptor component 1 (PGRMC1) contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (23 kDa) than predicted (21 kDa).
  • ICC/IF image of ab88381 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88381, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PGRMC1 antibody (ab88381)

ab88381 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88381.
Please use the links above to contact us or submit feedback about this product.