Anti-PGRMC1 antibody - C-terminal (ab194879)
Key features and details
- Rabbit polyclonal to PGRMC1 - C-terminal
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PGRMC1 antibody - C-terminal
See all PGRMC1 primary antibodies -
Description
Rabbit polyclonal to PGRMC1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human PGRMC1 aa 100 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_006658.1).
Database link: O00264 -
Positive control
- WB: HeLa, Jurkat, 293T and NIH3T3 whole cell lysates. IP: 293T cell lysate. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab194879 was affinity purified using an epitope specific to PGRMC1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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Notes |
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IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
Target
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Function
Receptor for progesterone. -
Tissue specificity
Widely expressed, with highest expression in liver and kidney. -
Sequence similarities
Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
Domain
The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 317706 Cow
- Entrez Gene: 100058718 Horse
- Entrez Gene: 10857 Human
- Entrez Gene: 53328 Mouse
- Entrez Gene: 100174262 Orangutan
- Entrez Gene: 396946 Pig
- Entrez Gene: 100357097 Rabbit
- Entrez Gene: 711792 Rhesus monkey
see all -
Alternative names
- HPR6.6 antibody
- Membrane associated progesterone receptor component 1 antibody
- Membrane-associated progesterone receptor component 1 antibody
see all
Images
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All lanes : Anti-PGRMC1 antibody - C-terminal (ab194879) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 30 secondsLysates in lanes 1-3 were prepared using RIPA lysis buffer. Lysates in lanes 4-5 were prepared using NETN lysis buffer.
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Detection of PGRMC1 in immunoprecipitates of 293T whole cell lysate prepared using RIPA lysis buffer (1 mg for IP, 20% of IP loaded) using ab194879 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling PGRMC1 with ab194879 at a 1/5000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab194879 has not yet been referenced specifically in any publications.