Detection of PGRMC2 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab104437 at 3 µg/mg lysate for IP. An anti-PGRMC2 antibody which recognizes a downstream epitope was used at 1 µg/ml for subsequent western blot detection.
Detection: chemiluminescence with exposure time of 10 seconds.
References for Anti-PGRMC2 antibody (ab104437)
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