PGRMC2 antibody (ab56711) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.
Western blot - PGRMC2 antibody (ab56711)
Predicted band size : 24 kDa PGRMC2 antibody (ab56711) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-PGRMC2 antibody (ab56711)
Overlay histogram showing HeLa cells stained with ab56711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-PGRMC2 antibody (ab56711)
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