Overview

  • Product nameAnti-PGRMC2 antibody
    See all PGRMC2 primary antibodies
  • Description
    Mouse monoclonal to PGRMC2
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: NGKVFDVTKG SKFYGPAGPY GIFAGRDASR GLATFCLDKD ALRDEYDDLS DLNAVQMESV REWEMQFKEK YDYVGRLLKP GEEPSEYTDE EDTKDHNKQD , corresponding to amino acids 124-224 of Human PGRMC2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceMembrane associated progesterone receptor component 2 (PGRMC2) belongs to the cytochrome b5 family. The molecular function is primarily steroid binding.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • DG6 antibody
    • Membrane associated progesterone receptor component 2 antibody
    • PMBP antibody
    • Progesterone membrane binding protein antibody
    • Progesterone receptor membrane component 2 antibody
    • Steroid receptor protein DG6 antibody
    see all

Anti-PGRMC2 antibody images

  • PGRMC2 antibody (ab56711) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 24 kDa
    PGRMC2 antibody (ab56711) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab56711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PGRMC2 antibody (ab56711)

ab56711 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"