Anti-PGRPS antibody [188C424] (ab13903)
Key features and details
- Mouse monoclonal [188C424] to PGRPS
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PGRPS antibody [188C424]
See all PGRPS primary antibodies -
Description
Mouse monoclonal [188C424] to PGRPS -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: YVLKGHRDVQRTLSPG , corresponding to amino acids 165-180 of Human PGRPS.
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Positive control
- IHC: Human esophagus cells. WB: Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
188C424 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa. |
Target
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Function
Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity. -
Tissue specificity
Highly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils. -
Sequence similarities
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. -
Post-translational
modificationsN-glycosylated. N-glycosylation is required for bactericidal activity. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Database links
- Entrez Gene: 8993 Human
- Omim: 604963 Human
- SwissProt: O75594 Human
- Unigene: 137583 Human
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Alternative names
- MGC126894 antibody
- MGC126896 antibody
- Peptidoglycan recognition protein 1 antibody
see all
Images
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Immunocytochemistry analysis of human esophagus cells labelling PGRPS with ab13903 at 20 µg/mL. The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining of peripheral leukocytes and neutrophils was observed. Staining was performed by Histowiz.
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Anti-PGRPS antibody [188C424] (ab13903) at 2 µg/ml + Jurkat cell lysate
Predicted band size: 22 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab13903 has been referenced in 1 publication.
- Dey S & Bishayi B Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation. J Inflamm (Lond) 13:36 (2016). IF . PubMed: 27932936