Anti-PGRPS antibody [188C424] (ab13903)

Overview

  • Product nameAnti-PGRPS antibody [188C424]
    See all PGRPS primary antibodies
  • Description
    Mouse monoclonal [188C424] to PGRPS
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: YVLKGHRDVQRTLSPG , corresponding to amino acids 165-180 of Human PGRPS.

  • Positive control
    • Tested with mouse spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesWB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity.
    • Tissue specificityHighly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils.
    • Sequence similaritiesBelongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
    • Post-translational
      modifications
      N-glycosylated. N-glycosylation is required for bactericidal activity.
    • Cellular localizationSecreted. Cytoplasmic granule.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC126894 antibody
      • MGC126896 antibody
      • Peptidoglycan recognition protein 1 antibody
      • Peptidoglycan recognition protein antibody
      • Peptidoglycan recognition protein short antibody
      • PGLYRP antibody
      • PGLYRP1 antibody
      • PGRP antibody
      • PGRP S antibody
      • PGRP-S antibody
      • PGRP1_HUMAN antibody
      • PHRP, short antibody
      • SBBI68 antibody
      • TAG7 antibody
      • TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein) antibody
      • TNFSF 3L antibody
      • TNFSF3L antibody
      • UNQ639/PRO1269 antibody
      see all

    Anti-PGRPS antibody [188C424] images

    • ab13903 at 1/100 staining human bone Marrow derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked with BSA before incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells were counterstained with DAPI.

      The image shows staining mainly in the cytoplasmic region.

      See Abreview

    • Anti-PGRPS antibody [188C424] (ab13903) at 2 µg/ml + Jurkat cell lysate

      Predicted band size : 22 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)

    References for Anti-PGRPS antibody [188C424] (ab13903)

    ab13903 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Bone Marrow derived CD34+ cells)
    Specification Bone Marrow derived CD34+ cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.25% Triton X-100 for 10min
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 29 2007

    Thank you for your enquiry. Unfortunately I do not have access to the Lui paper. According to the Swiss Prot reference for mouse the target is “strongly expressed in spleen”, this was the positive control that was used. The predicted molecular weig...

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    Thank you for your enquiry. This product will cross react with mouse, the data sheet has now been updated t reflect this. Western blot analysis was carried out on PGRP-S in tissue lysates from mouse spleen using Ab13903 at a dilution of 2 µg/ml. Thank ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"