Overview

  • Product nameAnti-PHACTR1 antibody
  • Description
    Rabbit polyclonal to PHACTR1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: QRPTAEELEQ RNILKPRNEQ EEQEEKREIK RRLTRKLSQR PTVEELRERK, corresponding to internal sequence amino acids 468-517 of Human PHACTR1 (NP_112210)

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePHACTR1 belongs to the phosphatase and actin regulator family. It binds PPP1CA and actin and contains 4 RPEL repeats. There are two named isoforms.
  • Cellular localizationCytoplasm. Cell junction; synapse.
  • Database links
  • Alternative names
    • 9630030F18Rik antibody
    • dJ257A7.2 antibody
    • KIAA1733 antibody
    • MGC112561 antibody
    • MGC126575 antibody
    • MGC126577 antibody
    • Phosphatase and actin regulator 1 antibody
    • RPEL antibody
    • RPEL repeat containing 1 antibody
    • RPEL1 antibody
    see all

Anti-PHACTR1 antibody images

  • Anti-PHACTR1 antibody (ab84646) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 66 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PHACTR1 with ab84646 at 5.0µg/ml.

References for Anti-PHACTR1 antibody (ab84646)

ab84646 has not yet been referenced specifically in any publications.

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I can confirm that lysates from non-transfected Jurkat cells were used to test this antibody (ab84646) for Western blot application.
PHACTR1 is expressed in bone marrow and lymph n...

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