Anti-PHACTR3 antibody - N-terminal (ab171301)

Overview

  • Product name
    Anti-PHACTR3 antibody - N-terminal
    See all PHACTR3 primary antibodies
  • Description
    Rabbit polyclonal to PHACTR3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PHACTR3 aa 24-54 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96KR7

  • Positive control
    • 293 cell lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab171301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.

Target

  • Tissue specificity
    Abundantly expressed in brain. Also found in several tumors such as lung carcinomas, nervous tumors and HL-60 leukemia cells. Isoform 3 is the major form in U937, GOTO and HL-60 leukemia cells.
  • Sequence similarities
    Belongs to the phosphatase and actin regulator family.
    Contains 4 RPEL repeats.
  • Cellular localization
    Nucleus matrix. Localized to the nuclear matrix-intermediate filament scaffold. Isoform 2 is also found in some cytoplasmic extensions.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf101 antibody
    • H17739 antibody
    • MGC117178 antibody
    • PHACTR 3 antibody
    • Phactr3 antibody
    • PHAR3_HUMAN antibody
    • Phosphatase and actin regulator 3 antibody
    • phosphatase and actin regulator 3, isoform 1 antibody
    • Scaffold associated PP1 inhibiting protein antibody
    • Scaffold-associated PP1-inhibiting protein antibody
    • SCAPIN 1 antibody
    • SCAPIN1 antibody
    • Scapinin antibody
    see all

Images

  • Anti-PHACTR3 antibody - N-terminal (ab171301) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PHACTR3 using ab171301 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171301 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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