Overview

  • Product name
    Phalloidin-iFluor 555 Reagent - CytoPainter
    See all F-actin kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Phalloidin-iFluor 555 Reagent | CytoPainter (ab176756) is one of a series of phalloidin conjugates that selectively binds to actin filaments, also known as F-actin. The iFluor 555 dye can be easily detected with a fluorescent microscope at Ex/Em = 556/574 nm. Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.


    Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.


     

  • Notes

    Actin is a globular, roughly 42-kDa protein found in almost all eukaryotic cells. It is also one of the most highly conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular processes including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, as well as the establishment and maintenance of cell junctions and cell shape.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 555 reagent (ab176755). Nuclei were stained with Nuclear Green DCS1 (ab138905).

  • Excitation and emission of phalloidin-iFluor 555 reagent.

Protocols

References

This product has been referenced in:
  • Li CX  et al. 'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice. Oncol Lett 13:177-183 (2017). Read more (PubMed: 28123538) »
  • Naranda J  et al. Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA). PeerJ 5:e3079 (2017). Read more (PubMed: 28344902) »

See all 3 Publications for this product

Customer reviews and Q&As

Phalloidin staining of paraffin embeded tissue.

Poor Excellent 5/5 (Ease of Use)
Abreviews
I purchased Phalloidin to stain paraffin embedded tissue, but it does not work. I tried many variations of time and temperature, concentration, with or without antigen retrieval, permeabilization and there was not a trace of fluorescent staining. Although as my positive control I used formalin fixed primary hepatocytes on chamber slides and that was very good.
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Submitted Aug 03 2017

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