Overview

  • Product nameAnti-PHAP antibody
    See all PHAP primary antibodies
  • Description
    Rabbit polyclonal to PHAP
  • SpecificityThis antibody cross-reacts with both PHAP I and PHAP III.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human).

  • Positive control
    • Raji whole cell lysate.
  • General notesStable for one year.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesIHC-P: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects bands of approximately 35 and 32 kDa corresponding to PHAPIII and PHAPI respectively. Two cross reacting bands are also observed below 32 kDa, which may be differentially spliced forms of PHAPI.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThis antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Acidic (leucine-rich) nuclear phosphoprotein 32 family, member B antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • ANP32A antibody
      • ANP32B antibody
      • I1PP2A antibody
      • LANP antibody
      • MAPM antibody
      • PHAP1 antibody
      • PHAPI2 antibody
      • PHAPIII antibody
      • PP32 antibody
      • Putative Human HLA Class II Associated Protein antibody
      see all

    Anti-PHAP antibody images

    • Immunofluorescence of PHAP in Human small intestine tissue with ab5987 at 20 ug/mL.



    • Predicted band size : 29 kDa

      Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1 µg/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.

      Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1 µg/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.
    • ab5987 at 10µg/ml staining PHAP in human small intestine tissue by IHC 

    References for Anti-PHAP antibody (ab5987)

    ab5987 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"