Overview

  • Product nameAnti-PHAP antibody
    See all PHAP primary antibodies
  • Description
    Rabbit polyclonal to PHAP
  • SpecificityThis antibody cross-reacts to all three identified isoforms of PHAP (PHAPI, PHAP12a and PHAPIII).
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human).

  • Positive control
    • Raji whole cell lysate.
  • General notesStable for one year.

Properties

Applications

Our Abpromise guarantee covers the use of ab5989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 35 kDa corresponding to PHAPIII(predicted molecular weight: 29 kDa). A band below 35kDa is also observed, consisting of a doublet of PHAPI and PHAP12a.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThis antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Acidic (leucine-rich) nuclear phosphoprotein 32 family, member B antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • ANP32A antibody
      • ANP32B antibody
      • I1PP2A antibody
      • LANP antibody
      • MAPM antibody
      • PHAP1 antibody
      • PHAPI2 antibody
      • PHAPIII antibody
      • PP32 antibody
      • Putative Human HLA Class II Associated Protein antibody
      see all

    Anti-PHAP antibody images



    • Predicted band size : 29 kDa


      Western blot analysis of PHAP expression in human Raji cell lysate with anti- PHAP at 1 µg/ml. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).
    • ab5989 at 1µg/ml staining PHAP in Raji cells by ICC/IF

    References for Anti-PHAP antibody (ab5989)

    ab5989 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"