Overview

  • Product name
  • Description
    Rabbit polyclonal to PHAP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 149-163 of Human I1 alpha PP2A.

Properties

Applications

Our Abpromise guarantee covers the use of ab1181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Reactive with I1aPP2A from diverse tissue sources by Western blotting. Used at 1:100 with cell extracts.
    Other immunodetection procedures not tested.
  • Target

    • Function
      Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
    • Tissue specificity
      Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
    • Sequence similarities
      Belongs to the ANP32 family.
      Contains 4 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
      The N-terminus is blocked.
      Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    • Cellular localization
      Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • AN32A_HUMAN antibody
      • ANP32A antibody
      • C15orf1 antibody
      • Cerebellar leucine rich acidic nuclear protein antibody
      • Hepatopoietin Cn antibody
      • HPPCn antibody
      • I1PP2A antibody
      • Inhibitor 1 of protein phosphatase 2A antibody
      • inhibitor-1 of protein phosphatase-2A antibody
      • Lanp antibody
      • Leucine rich acidic nuclear protein antibody
      • Leucine-rich acidic nuclear protein antibody
      • MAPM antibody
      • Mapmodulin antibody
      • MGC119787 antibody
      • MGC150373 antibody
      • PHAPI antibody
      • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
      • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
      • PP32 antibody
      • Putative HLA DR associated protein I antibody
      • Putative HLA-DR-associated protein I antibody
      • Putative human HLA class II associated protein I antibody
      • Putative human HLA class II-associated protein antibody
      see all

    Images

    • Western blot of 10 µg HEK 293 cell extracts with Anti- I1α (GLO149). The band corresponds to I1αPP2A (~ 30 kDa).

    References

    ab1181 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    I have confirmed with the laboratory that the conditions you have used to prepare the cell lysates seem appropriate and similar to what we use and the blocking agent seems to be appropriate. The difference we observe in the protocols is the incubati...

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    Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab1181. Could you please clarify a few more details so I can understand your problem better and resolve it? 1) what was the sample preparati...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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