Overview

  • Product nameAnti-PHAPI2 / APRIL antibody
    See all PHAPI2 / APRIL primary antibodies
  • Description
    Goat polyclonal to PHAPI2 / APRIL
  • SpecificityThis antibody does NOT recognize the TNF family member also known as APRIL - LocusLink Number 8741
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: KRKRETDDEGEDD, corresponding to C terminal amino acids 237-249 of Human PHAPI2/ APRIL.

  • Positive control
    • Human Tonsil lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human PHAPI2 / APRIL peptide (ab23013).

Target

  • FunctionMultifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
  • Tissue specificityExpressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.
  • Sequence similaritiesBelongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • DomainHistone binding is mediated by the concave surface of the LRR region.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localizationNucleus. Accumulates in the nuclei at the S phase and Cytoplasm. Lacks a nuclear localization signal.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic (leucine rich) nuclear phosphoprotein 32 family member B antibody
    • Acidic leucine rich nuclear phosphoprotein 32 family member B antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member B antibody
    • Acidic nuclear phosphoprotein 32 family member B antibody
    • Acidic protein rich in leucines antibody
    • AN32B_HUMAN antibody
    • ANP 32B antibody
    • ANP32B antibody
    • APRIL antibody
    • OTTHUMP0000006377 antibody
    • PAL 31 antibody
    • PAL31 antibody
    • PHAPI 2 antibody
    • PHAPI2 antibody
    • PHAPI2 protein antibody
    • PHAPI2a antibody
    • Proliferation related acidic leucine rich protein antibody
    • Putative HLA-DR-associated protein I-2 antibody
    • Silver stainable protein SSP 29 antibody
    • Silver stainable protein SSP29 antibody
    • Silver-stainable protein SSP29 antibody
    • Ssp 29 antibody
    • Ssp29 antibody
    see all

Anti-PHAPI2 / APRIL antibody images

  • All lanes : Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml

    Lane 1 : Human tonsil lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : Pig spleen lysate

    Lysates/proteins at 35 mg/ml per lane.


    Predicted band size : 29 kDa
    Observed band size : 28-30 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml + NIH3T3 cell lysate at 35 µg

    Predicted band size : 29 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.



  • Predicted band size : 29 kDa
    ab4224 at a concentration of 1 µg/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30 µg total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL). ab4224 at a concentration of 1 µg/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30 µg total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL).
  • Anti-PHAPI2 / APRIL antibody (ab4224) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ab4224 (3.8µg/ml) staining of paraffin embedded Human Breast tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in lobular cells.

References for Anti-PHAPI2 / APRIL antibody (ab4224)

This product has been referenced in:
  • Khan AP  et al. Quantitative proteomic profiling of prostate cancer reveals a role for miR-128 in prostate cancer. Mol Cell Proteomics 9:298-312 (2010). WB ; Human . Read more (PubMed: 19955085) »
  • Munemasa Y  et al. Promoter region-specific histone incorporation by the novel histone chaperone ANP32B and DNA-binding factor KLF5. Mol Cell Biol 28:1171-81 (2008). WB ; Human . Read more (PubMed: 18039846) »

See all 2 Publications for this product

Product Wall

Thanks for your email. What size band did you see with the GST fusion protein? As I mentioned, we do recommend using human lymph node lysate as a positive control with this antibody. To our knowledge it has not been tested with HeLa cells, I don't know...

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Thank you for your email. At what dilutions of ab4224 did you see a signal with the GST-APRIL purified protein? Ab4224 was characterized using Human Lymph Node lysate, which is recommended as a positive control. You didn't specifically mention which h...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab4224. I do need a few more details from you in order to investigate this problem. What dilutions of ab4224 have you tried? Is your secondary antibody worki...

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Thank you for your enquiry. We are still selling the first batch - all data on the datasheet is therefore correct.

Thank you for this feedback information!

We are very sorry but we can't provide more technical support in this matter. This antibody was produced by another company and the information we have is very limited. We have do not have other vials from a different batch, so either we can offer ...

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Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. The question we would need to ask is whether the HA tag could be blocking the epitope ie is it on the C-terminus or the N-terminus of the fusion prote...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"