Overview

  • Product nameAnti-PHAX antibody
  • Description
    Rabbit polyclonal to PHAX
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human PHAX.

  • Positive control
    • HT-29 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.

Target

  • FunctionA phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
  • Sequence similaritiesBelongs to the PHAX family.
  • Post-translational
    modifications
    Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > Cajal body. Cytoplasm. Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • PHAX antibody
    • PHAX_HUMAN antibody
    • Phosphorylated adapter RNA export protein antibody
    • RNA U small nuclear RNA export adapter protein antibody
    • RNUXA antibody
    see all

Anti-PHAX antibody images

  • All lanes : Anti-PHAX antibody (ab110525) at 1/500 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : HT-29 cell lysate with blocking peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 44 kDa

References for Anti-PHAX antibody (ab110525)

ab110525 has not yet been referenced specifically in any publications.

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