Overview

  • Product name
  • Description
    Mouse polyclonal to PHC3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full-length human PHC3, (AAH22325, 151 a.a)

  • Positive control
    • PHC3 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight for Isoform 5: 16 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
    • Sequence similarities
      Contains 1 FCS-type zinc finger.
      Contains 1 SAM (sterile alpha motif) domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early development regulator 3 antibody
      • Early development regulatory protein 3 antibody
      • EDR3 antibody
      • Homolog of polyhomeotic 3 antibody
      • HPH 3 antibody
      • hPH3 antibody
      • PH3 antibody
      • PHC 3 antibody
      • Phc3 antibody
      • PHC3_HUMAN antibody
      • Polyhomeotic homolog 3 (Drosophila) antibody
      • Polyhomeotic homolog 3 antibody
      • Polyhomeotic-like protein 3 antibody
      see all

    Images

    • All lanes : Anti-PHC3 antibody (ab69142) at 1/500 dilution

      Lane 1 : PHC3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa

    References

    This product has been referenced in:
    • Quijano JC  et al. Wg signaling via Zw3 and mad restricts self-renewal of sensory organ precursor cells in Drosophila. Genetics 189:809-24 (2011). Read more (PubMed: 21868604) »

    See 1 Publication for this product

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    There are currently no Customer reviews or Questions for ab69142.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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