Overview

  • Product nameAnti-PHC3 antibody
    See all PHC3 primary antibodies
  • Description
    Rabbit polyclonal to PHC3
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human, Drosophila melanogaster
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human PHC3 aa 700-750. The exact sequence is proprietary.
    Database link: AAI31773.1

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified using an epitope specific to PHC3 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab80612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22019301
WB 1/2000 - 1/10000. Predicted molecular weight: 106 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similaritiesContains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early development regulator 3 antibody
    • Early development regulatory protein 3 antibody
    • EDR3 antibody
    • Homolog of polyhomeotic 3 antibody
    • HPH 3 antibody
    • hPH3 antibody
    • PH3 antibody
    • PHC 3 antibody
    • Phc3 antibody
    • PHC3_HUMAN antibody
    • Polyhomeotic homolog 3 (Drosophila) antibody
    • Polyhomeotic homolog 3 antibody
    • Polyhomeotic-like protein 3 antibody
    see all

Anti-PHC3 antibody images

  • All lanes : Anti-PHC3 antibody (ab80612) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 106 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate using ab80612 at 3µg/mg lysate (lane 1) or a control IgG (lane 2). Bands were detected by Western blot using ab80612 at 1µg/ml. Chemiluminescence with exposure times of 3 minutes.

References for Anti-PHC3 antibody (ab80612)

This product has been referenced in:
  • Datta RR  et al. A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity. Dev Biol 360:391-402 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22019301) »
  • Datta RR  et al. A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity. Dev Biol 360:391-402 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22019301) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Cell (heart cryosection tissue)
Specification heart cryosection tissue
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
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Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"