Recombinant
RabMAb

Anti-PHC3 antibody [EPR13306] (ab180577)

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Overview

  • Product name
    Anti-PHC3 antibody [EPR13306]
    See all PHC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR13306] to PHC3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHC3 aa 300-400. The exact sequence is proprietary.
    Database link: Q8NDX5

  • Positive control
    • HepG2, SH-SY5Y, HeLa and 293 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13306
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similarities
    Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Early development regulator 3 antibody
      • Early development regulatory protein 3 antibody
      • EDR3 antibody
      • Homolog of polyhomeotic 3 antibody
      • HPH 3 antibody
      • hPH3 antibody
      • PH3 antibody
      • PHC 3 antibody
      • Phc3 antibody
      • PHC3_HUMAN antibody
      • Polyhomeotic homolog 3 (Drosophila) antibody
      • Polyhomeotic homolog 3 antibody
      • Polyhomeotic-like protein 3 antibody
      see all

    Images

    • Western blot - Anti-PHC3 [EPR13306] antibody (ab180577)
      Western blot - Anti-PHC3 [EPR13306] antibody (ab180577)
      All lanes : Anti-PHC3 antibody [EPR13306] (ab180577) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 106 kDa

    References

    ab180577 has not yet been referenced specifically in any publications.

    Publishing research using ab180577? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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