Recombinant
RabMAb

Anti-PHC3 antibody [EPR13306] (ab180577)

Overview

  • Product name
    Anti-PHC3 antibody [EPR13306]
    See all PHC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR13306] to PHC3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PHC3 aa 300-400. The exact sequence is proprietary.
    Database link: Q8NDX5

  • Positive control
    • HepG2, SH-SY5Y, HeLa and 293 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13306
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Sequence similarities
    Contains 1 FCS-type zinc finger.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early development regulator 3 antibody
    • Early development regulatory protein 3 antibody
    • EDR3 antibody
    • Homolog of polyhomeotic 3 antibody
    • HPH 3 antibody
    • hPH3 antibody
    • PH3 antibody
    • PHC 3 antibody
    • Phc3 antibody
    • PHC3_HUMAN antibody
    • Polyhomeotic homolog 3 (Drosophila) antibody
    • Polyhomeotic homolog 3 antibody
    • Polyhomeotic-like protein 3 antibody
    see all

Images

  • All lanes : Anti-PHC3 antibody [EPR13306] (ab180577) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 106 kDa

References

ab180577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180577.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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