Anti-PHD finger protein 6 antibody (ab99395)

Overview

  • Product nameAnti-PHD finger protein 6 antibody
    See all PHD finger protein 6 primary antibodies
  • Description
    Rabbit polyclonal to PHD finger protein 6
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 315-365 of Human PHD finger protein 6 (NP_115834.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99395 was affinity purified using an epitope specific to PHD finger protein 6 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in transcriptional regulation.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseDefects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • Sequence similaritiesContains 2 PHD-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC004383.6 antibody
    • BFLS antibody
    • BORJ antibody
    • CENP 31 antibody
    • Centromere protein 31 antibody
    • MGC14797 antibody
    • OTTHUMP00000024063 antibody
    • PHD finger protein 6 antibody
    • PHD like zinc finger protein antibody
    • PHD-like zinc finger protein antibody
    • Phf6 antibody
    • PHF6_HUMAN antibody
    see all

Anti-PHD finger protein 6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling PHD finger protein 6 with ab99395 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PHD finger protein 6 antibody (ab99395) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 3 seconds
  • Detection of PHD finger protein 6 by Western Blot of Immunprecipitate.
    ab99395 at 1µg/ml staining PHD finger protein 6 in HeLa whole cell lysate immunoprecipitated using ab99395 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-PHD finger protein 6 antibody (ab99395)

ab99395 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"