Anti-PHD finger protein 6 antibody (ab99398)

Overview

  • Product nameAnti-PHD finger protein 6 antibody
    See all PHD finger protein 6 primary antibodies
  • Description
    Rabbit polyclonal to PHD finger protein 6
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human PHD finger protein 6 (NP_115834.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in transcriptional regulation.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseDefects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • Sequence similaritiesContains 2 PHD-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC004383.6 antibody
    • BFLS antibody
    • BORJ antibody
    • CENP 31 antibody
    • Centromere protein 31 antibody
    • MGC14797 antibody
    • OTTHUMP00000024063 antibody
    • PHD finger protein 6 antibody
    • PHD like zinc finger protein antibody
    • PHD-like zinc finger protein antibody
    • Phf6 antibody
    • PHF6_HUMAN antibody
    see all

Anti-PHD finger protein 6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PHD finger protein 6 with ab99398 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PHD finger protein 6 antibody (ab99398) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 10 seconds
  • Detection of PHD finger protein 6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99398 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with exposure time of 3 seconds.

References for Anti-PHD finger protein 6 antibody (ab99398)

ab99398 has not yet been referenced specifically in any publications.

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