• Product nameAnti-PHD1/prolyl hydroxylase antibody
    See all PHD1/prolyl hydroxylase primary antibodies
  • Description
    Mouse polyclonal to PHD1/prolyl hydroxylase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Caenorhabditis elegans
  • Immunogen

    Recombinant fusion protein: RNPHEVMPVF RHRFAITIWY MDKSERDKAL AKGKESDAAC, corresponding to amino acids 545-644 of Caenorhabditis elegans PHD1

  • General notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.



Our Abpromise guarantee covers the use of ab52726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 44 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • Tissue specificityExpressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Alternative names
    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

Anti-PHD1/prolyl hydroxylase antibody images

  • All lanes : Anti-PHD1/prolyl hydroxylase antibody (ab52726) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

    Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-PHD1/prolyl hydroxylase antibody (ab52726)

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab52726.
Please use the links above to contact us or submit feedback about this product.