Overview

  • Product nameAnti-PHD1/prolyl hydroxylase antibody
    See all PHD1/prolyl hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to PHD1/prolyl hydroxylase
  • SpecificityAntibody was raised against isoform CRA_a of PHD1/prolyl hydroxylase.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 (MCLPSPSKPT SLHPCQAMVA CYPGNGLGYV RHVDNPHGDG RCITCIYYLN) of Human PHD1/prolyl hydroxylase (EAW57007).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • Tissue specificityExpressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

Anti-PHD1/prolyl hydroxylase antibody images

  • Anti-PHD1/prolyl hydroxylase antibody (ab86980) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Observed band size : ~16 kDa (why is the actual band size different from the predicted?)

References for Anti-PHD1/prolyl hydroxylase antibody (ab86980)

This product has been referenced in:
  • Gilbert JS  et al. Exercise training attenuates placental ischemia-induced hypertension and angiogenic imbalance in the rat. Hypertension 60:1545-51 (2012). WB ; Rat . Read more (PubMed: 23090773) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"