Recombinant
RabMAb

Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Overview

  • Product name
    Anti-PHD1/prolyl hydroxylase antibody [EPR2746]
    See all PHD1/prolyl hydroxylase primary antibodies
  • Description
    Rabbit monoclonal [EPR2746] to PHD1/prolyl hydroxylase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PHD1/prolyl hydroxylase aa 350-450. The exact sequence is proprietary.

  • Positive control
    • WB: A549, HeLa and MCF-7 cell lysates IHC-P: Human breast carcinoma tissue and Human lung carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
ICC 1/50 - 1/100.
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
    • Tissue specificity
      Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
    • Sequence similarities
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp434E026 antibody
      • EGL nine (C.elegans) homolog 2 antibody
      • Egl nine homolog 2 (C. elegans) antibody
      • Egl nine homolog 2 antibody
      • EGLN 2 antibody
      • EGLN2 antibody
      • EGLN2_HUMAN antibody
      • EIT 6 antibody
      • EIT6 antibody
      • Estrogen-induced tag 6 antibody
      • HIF P4H 1 antibody
      • HIF PH1 antibody
      • HIF prolyl hydroxylase 1 antibody
      • HIF-PH1 antibody
      • HIF-prolyl hydroxylase 1 antibody
      • HIFPH 1 antibody
      • HIFPH1 antibody
      • HPH 3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • HPH3 antibody
      • Hypoxia inducible factor prolyl hydroxylase 1 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
      • P4H1 antibody
      • PHD 1 antibody
      • PhD1 antibody
      • prolyl hydroxylase domain containing protein 1 antibody
      • Prolyl hydroxylase domain-containing protein 1 antibody
      see all

    Images

    • All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.

    References

    ab113077 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MKN7, MKN45, SNU-1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    MKN7, MKN45, SNU-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 20 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (osteosarcoma cells)
    Specification
    osteosarcoma cells
    Treatment
    control and siRNA for PHD1
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    Submitted Feb 13 2015

    Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during a week.

    We recently undertook an in-depth study examining storage conditions and antibody performa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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