Anti-PHD2 / prolyl hydroxylase antibody (ab26058)

Overview

  • Product nameAnti-PHD2 / prolyl hydroxylase antibody
    See all PHD2 / prolyl hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to PHD2 / prolyl hydroxylase
  • Specificityab26058 recognises PHD2/prolyl hydroxylase.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: QPAYATRYAI TVWYFDADER ARAKVKYLTG EKGVRVELNK PSDSVGKDVF, corresponding to C terminal amino acids 377-426 of Human PHD2/ prolyl hydroxylase

  • Positive control
    • HepG2 cell lysate for Western blot.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab26058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificityAccording to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
  • Involvement in diseaseDefects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
    Contains 1 MYND-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1ORF12 antibody
    • Chromosome 1 Open Reading Frame 12 antibody
    • DKFZp761F179 antibody
    • ECYT 3 antibody
    • ECYT3 antibody
    • EGL 9 homolog of C. elegans 1 antibody
    • EGL nine (C.elegans) homolog 1 antibody
    • Egl nine homolog 1 (C. elegans) antibody
    • Egl nine homolog 1 antibody
    • Egl nine like protein 1 antibody
    • EGLN 1 antibody
    • Egln1 antibody
    • EGLN1_HUMAN antibody
    • HIF PH2 antibody
    • HIF Prolyl Hydroxylase 2 antibody
    • HIF-PH2 antibody
    • HIF-prolyl hydroxylase 2 antibody
    • HIFP4H 2 antibody
    • HIFPH2 antibody
    • HPH 2 antibody
    • HPH-2 antibody
    • HPH2 antibody
    • Hypoxia inducible factor prolyl hydroxylase 2 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 2 antibody
    • ORF13 antibody
    • P4H2 antibody
    • PHD 2 antibody
    • PhD2 antibody
    • PNAS 118 antibody
    • PNAS 137 antibody
    • Prolyl Hydroxylase Domain Containing Protein 2 antibody
    • Prolyl hydroxylase domain-containing protein 2 antibody
    • Rat Homolog of SM20 antibody
    • SM 20 antibody
    • SM-20 antibody
    • SM20 antibody
    • Zinc finger MYND domain containing protein 6 antibody
    • ZMYND6 antibody
    see all

Anti-PHD2 / prolyl hydroxylase antibody images

  • Anti-PHD2 / prolyl hydroxylase antibody (ab26058) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa

References for Anti-PHD2 / prolyl hydroxylase antibody (ab26058)

This product has been referenced in:
  • Tennant DA & Gottlieb E HIF prolyl hydroxylase-3 mediates alpha-ketoglutarate-induced apoptosis and tumor suppression. J Mol Med 88:839-49 (2010). WB ; Human . Read more (PubMed: 20383689) »

See 1 Publication for this product

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DISCOUNT CODE: ***
Expiration date: October 5, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab26058 and include this code in the “Additional Com...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"