Overview

  • Product name
  • Description
    Goat polyclonal to PHD3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    C-RLGKYYVKERSK

    , corresponding to internal sequence amino acids 108-119 of Human PHD3 (NP_071356.1).

  • Positive control
    • human Muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/8,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
    • Tissue specificity
      Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
    • Sequence similarities
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Egl 9 family hypoxia inducible factor 3 antibody
      • Egl nine homolog 3 (C. elegans) antibody
      • Egl nine homolog 3 antibody
      • Egl nine like protein 3 isoform antibody
      • EGL9 homolog of C. elegans 3 antibody
      • EGLN3 antibody
      • EGLN3_HUMAN antibody
      • Factor responsive smooth muscle protein antibody
      • HIF Prolyl Hydroxylase 3 antibody
      • HIF-PH3 antibody
      • HIF-prolyl hydroxylase 3 antibody
      • HIFP4H3 antibody
      • HIFPH3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 3 antibody
      • P4H3 antibody
      • PHD3 antibody
      • Prolyl Hydroxylase Domain Containing Protein 3 antibody
      • Prolyl hydroxylase domain-containing protein 3 antibody
      • SM20 antibody
      see all

    Images

    • Anti-PHD3 antibody (ab77397) at 0.1 µg/ml + Human Muscle lysate (in RIPA buffer) at 35 µg

      Predicted band size : 27 kDa
      Observed band size : 27 kDa

    References

    ab77397 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77397.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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