Overview

  • Product nameAnti-PHD3 antibody
    See all PHD3 primary antibodies
  • Description
    Mouse polyclonal to PHD3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full length human PHD3, amino acids 1-239 (NP_071356.1).

  • Positive control
    • PHD3 transfected 293T cell lysate, human placenta tissue lysate and HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
  • Tissue specificityWidely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Egl 9 family hypoxia inducible factor 3 antibody
    • Egl nine homolog 3 (C. elegans) antibody
    • Egl nine homolog 3 antibody
    • Egl nine like protein 3 isoform antibody
    • EGL9 homolog of C. elegans 3 antibody
    • EGLN3 antibody
    • EGLN3_HUMAN antibody
    • Factor responsive smooth muscle protein antibody
    • HIF Prolyl Hydroxylase 3 antibody
    • HIF-PH3 antibody
    • HIF-prolyl hydroxylase 3 antibody
    • HIFP4H3 antibody
    • HIFPH3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 3 antibody
    • P4H3 antibody
    • PHD3 antibody
    • Prolyl Hydroxylase Domain Containing Protein 3 antibody
    • Prolyl hydroxylase domain-containing protein 3 antibody
    • SM20 antibody
    see all

Anti-PHD3 antibody images

  • Anti-PHD3 antibody (ab88582) at 1 µg + human placenta tissue lysate at 50 µg

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • All lanes : Anti-PHD3 antibody (ab88582) at 1 µg/ml

    Lane 1 : PHD3 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • ab88582 at 10µg/ml staining PHD3 in HeLa cells by Immunofluorescence.

References for Anti-PHD3 antibody (ab88582)

ab88582 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"