Overview

  • Product name
    Anti-PHD3 antibody [EG188e/d5]
    See all PHD3 primary antibodies
  • Description
    Mouse monoclonal [EG188e/d5] to PHD3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: MPLGHIMRLD LEKIALEYIV PCLHEVGFCY LDNFLGEVVG DCVLERVKQL HCTGALRDGQ LAGPRAGVSK RHLRGDQITW IGGNEEGCEA ISFLLSLIDR , corresponding to amino acids 1-100 of Human PHD3

Properties

Applications

Our Abpromise guarantee covers the use of ab77610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
  • Tissue specificity
    Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
  • Sequence similarities
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Egl 9 family hypoxia inducible factor 3 antibody
    • Egl nine homolog 3 (C. elegans) antibody
    • Egl nine homolog 3 antibody
    • Egl nine like protein 3 isoform antibody
    • EGL9 homolog of C. elegans 3 antibody
    • EGLN3 antibody
    • EGLN3_HUMAN antibody
    • Factor responsive smooth muscle protein antibody
    • HIF Prolyl Hydroxylase 3 antibody
    • HIF-PH3 antibody
    • HIF-prolyl hydroxylase 3 antibody
    • HIFP4H3 antibody
    • HIFPH3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 3 antibody
    • P4H3 antibody
    • PHD3 antibody
    • Prolyl Hydroxylase Domain Containing Protein 3 antibody
    • Prolyl hydroxylase domain-containing protein 3 antibody
    • SM20 antibody
    see all

References

This product has been referenced in:
  • Chan MC  et al. Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. J Biol Chem 291:20661-73 (2016). Human . Read more (PubMed: 27502280) »
  • Soilleux EJ  et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology 47:602-10 (2005). Read more (PubMed: 16324198) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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