Anti-PHD4/prolyl hydroxylase antibody (ab169483)

Overview

  • Product name
    Anti-PHD4/prolyl hydroxylase antibody
    See all PHD4/prolyl hydroxylase primary antibodies
  • Description
    Mouse polyclonal to PHD4/prolyl hydroxylase
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein, corresponding to amino acids 1-563 of Human PHD4/prolyl hydroxylase isoform 3 (NP_808807.2).

  • Positive control
    • PHD4/prolyl hydroxylase transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab169483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

Target

  • Function
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificity
    Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
  • Sequence similarities
    Contains 2 EF-hand domains.
    Contains 1 Fe2OG dioxygenase domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGLN4 antibody
    • HIF PH4 antibody
    • HIF Prolyl Hydroxylase 4 antibody
    • HIF prolyl hydroxylase PH 4 antibody
    • HIF prolyl hydroxylase PH4 antibody
    • HIF-PH4 antibody
    • HIF-prolyl hydroxylase 4 antibody
    • HIFPH4 antibody
    • HPH 4 antibody
    • HPH-4 antibody
    • Hypoxia inducible factor prolyl 4 hydroxylase antibody
    • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
    • P4H with transmembrane domain antibody
    • P4H-TM antibody
    • P4htm antibody
    • P4HTM_HUMAN antibody
    • PH 4 antibody
    • PH4 antibody
    • PHD 4 antibody
    • Proline 4 hydroxylase antibody
    • prolyl 4-hydroxylase antibody
    • Transmembrane prolyl 4-hydroxylase antibody
    see all

Images

  • All lanes : Anti-PHD4/prolyl hydroxylase antibody (ab169483) at 1 µg/ml

    Lane 1 : PHD4/prolyl hydroxylase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 63 kDa

References

ab169483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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