Overview

  • Product nameAnti-PHEMX antibody
  • Description
    Rabbit polyclonal to PHEMX
  • Specificityab65539 reacts with PHEMX isoform 2.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse
  • Immunogen

    A region within synthetic peptide: QGEEAAREDC LQGIRSFLRT HQQVASSLTS IGLALTVSAL LFSSFLWFAI, corresponding to internal sequence amino acids 144-193 of Human PHEMX

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePHEMX belongs to the conserved tetraspanin family of integral membrane proteins, which are thought to form multiprotein networks in the cell membrane in association with multiple protein partners. Tetraspanins have functional roles in cell motility, membrane fusion, proliferation, and adaptive immunity. This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. PHEMX may play a role in malignancies and disease that involve this region as well as hematopoietic cell function. There are 5 isoforms produced by alternative splicing.
  • Cellular localizationMembrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • ART1 antibody
    • FLJ17158 antibody
    • FLJ97586 antibody
    • MGC22455 antibody
    • Pan hematopoietic expression antibody
    • PHEMX antibody
    • PHMX antibody
    • tetraspanin 32 antibody
    • Tetraspanin antibody
    • tetraspanin32 antibody
    • TSPAN32 antibody
    • TSSC6 antibody
    • Tumor suppressing STF cDNA 6 antibody
    • Tumor suppressing subchromosomal transferable fragment cDNA 6 antibody
    • Tumor suppressing subtransferable candidate 6 antibody
    see all

Anti-PHEMX antibody images

  • Anti-PHEMX antibody (ab65539) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

References for Anti-PHEMX antibody (ab65539)

ab65539 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"