The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
PHEMX belongs to the conserved tetraspanin family of integral membrane proteins, which are thought to form multiprotein networks in the cell membrane in association with multiple protein partners. Tetraspanins have functional roles in cell motility, membrane fusion, proliferation, and adaptive immunity. This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. PHEMX may play a role in malignancies and disease that involve this region as well as hematopoietic cell function. There are 5 isoforms produced by alternative splicing.