Phenylalanine Assay Kit (ab83376)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Overview
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Product name
Phenylalanine Assay Kit -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Range
2 µM - 20 µM -
Assay time
1h 00m -
Product overview
Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.
In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Phenylalanine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K572 Phenylalanine Fluorometric Assay Kit. K572-100 is the same size as the 100 test size of ab83376.
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Phenyalanine Standard 1 vial Fructose Assay Buffer 1 x 25ml Phenylalanine Developer Mix 1 vial Phenylalanine Enzyme Mix 1 vial Tyrosinase 1 vial -
Research areas
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Relevance
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. -
Alternative names
- Phe
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab83376 has not yet been referenced specifically in any publications.